Use of low ionic strength saline for crossmatching and antibody screening.

نویسندگان

  • J A Lown
  • A L Barr
  • R E Davis
چکیده

A totally revised crossmatching and antibody screening procedure has been designed using low ionic strength saline as the suspending medium for red cells in the saline and enzyme phases as well as the antiglobulin test. The replacement of Pasteur pipettes with precision samplers for all serum and red cell dispensing has resulted in improvement in the reproducibility and standardisation of technique. The major advantages are improved sensitivity, shortened incubation time, and simplicity of technical performance.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

SENSITIBITY OF VARIOUS METHODS IN THE DETECTION OF IgG ANTIBODIES TO RED CELL ANTIGENS

In recent years, new methods such as microtube column agglutination methods and microplate methods using automated instruments have been used for blood typing, antibody screening testing and crossmatching testing. However, no precise comparison study of the performance of these methods and conventional tube methods has been done. In the present study, we performed a comparison study on sensitiv...

متن کامل

Frequencies and Specificities of “Enzyme-Only” Detected Erythrocyte Alloantibodies in Patients Hospitalized in Austria: Is an Enzyme Test Required for Routine Red Blood Cell Antibody Screening?

UNLABELLED The aim of this study was to determine the frequencies and specificities of "enzyme-only" detected red blood cell (RBC) alloantibodies in the routine antibody screening and antibody identification in patients hospitalized in Austria. Routine blood samples of 2420 patients were investigated. The antibody screening was performed with a 3-cell panel in the low-ionic strength saline- (LI...

متن کامل

Analysis of parameters affecting the hemagglutination activity of Escherichia coli possessing colonization factor antigens: improved medium for observing erythrocyte agglutination.

The hemagglutination (HA) activity of two strains of Escherichia coli, each possessing different colonization factor antigens (CFA), was examined under different test conditions. The effects of ionic strength, temperature, pH, cations, and reaction surface on erythrocyte (RBC) agglutination were analyzed. Strain H-10407 (CFA/I) caused the agglutination of human, bovine, and chicken RBC, whereas...

متن کامل

Improved antibody detection by the use of range expansion and longer filter wavelength in a low ionic strength-protamine sulphate Auto-Analyzer system.

Range expansion, achieved by insertion of a variable resistance between the colorimeter and the recorder together with the use of 550 nm colorimeter filters, has resulted in markedly improved sensitivity for antibody detection, and improved sample identification, in a low ionic strength-protamine sulphate (LISPS) system. Range expansion also permits a lower concentration of red cells to be used...

متن کامل

Significance of physicochemical factors in the transmission of Escherichia coli and chloride

Background: Organic manures are the source of many pathogenic bacteria which could be dangerous for human health. Bacterial transmission and retention in soil is important for processes ranging from contaminant degradation during in situ bioremediation to transport of pathogenic bacteria into groundwater. Methods: The aim of this study was to evaluate the transport of Escherichia coli and chl...

متن کامل

ذخیره در منابع من

ذخیره در منابع من قبلا به منابع من ذحیره شده

{@ msg_add @}


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Journal of clinical pathology

دوره 32 10  شماره 

صفحات  -

تاریخ انتشار 1979